冬虫夏草人工繁育品、野生品及亚香棒虫草中氨基酸比较

目的：比较冬虫夏草人工繁育品、野生品与亚香棒虫草中水解和游离氨基酸的含量。方法：采用氨基酸分析仪测定冬虫夏草人工繁育品、野生品以及亚香棒虫草中17种氨基酸的含量。结果：冬虫夏草人工繁育品的水解氨基酸含量为16.776%~19.080%,野生冬虫夏草为14.857%~21.959%,亚香棒虫草为13.043%~14.933%。冬虫夏草人工繁育品的游离氨基酸含量为1.767%~2.373%,野生冬虫夏草为1.753%~2.521%,亚香棒虫草为2.856%~3.197%。结论：冬虫夏草人工繁育品和野生品中氨基酸含量基本一致,和亚香棒虫草有显著性差异。本研究为冬虫夏草的鉴别及人工繁育品的进一步开发利用提供科学依据。     

四川松潘县秦艽资源调查及鉴别

目的调查松潘县秦艽的种类、资源分布、生态环境特征、生长状况和利用现状。方法实地踏查、样方调查结合文献研究。结果松潘县秦艽资源主要为秦艽Gentiana macrophylla Pall.、麻花秦艽Gentiana straminea Maxim.和粗茎秦艽Gentiana crassicaulis Duthie ex Burk.3种植物,境内各乡镇都有分布,尤以松潘中部的高半山和西北部的高原草甸分布为多。结论松潘县秦艽资源丰富,未能合理发展及利用,同时资源保护意识薄弱,大量秦艽资源因过度放牧而破坏。     

Epidemiological study of hepatitis E virus infection in the general population of Okinawa,Kyushu, Japan

Background and Aim: The aim of this study was to investigate the prevalence of hepatitis E virus (HEV) infection in the general population of Japan by determining presence of the antibody to HEV (anti‐HEV). Methods: The prevalence of HEV infection was determined by positivity of serum antibody to HEV (anti‐HEV). Results: On retrospective analysis, a significant decrease in anti‐HEV prevalence was found in Okinawa healthy residents from 1995 (15.8%) to 2005 (5.5%) (P < 0.0001). In 2005, the anti‐HEV prevalence was significantly higher in Okinawa wild boar hunters (25.3%) than in the residents (male 7.7% and female 4.1%) (P < 0.0001). A significant difference was found in the history of consumption of undercooked or raw boar meat between anti‐HEV positive and negative hunters (100% vs 64.3%) (P = 0.0018). Conclusions: In conclusion, the anti‐HEV prevalence has decreased in the residents of this area, but HEV infection has continued at a high rate in the hunters through the custom of eating undercooked or raw boar meat.     

广视野系统在复杂视网膜脱离手术中的应用

目的 :探讨广视野镜倒像装置系统在玻璃体视网膜显微手术治疗复杂视网膜脱离中应用的价值。方法 :将广视野镜倒像装置系统应用于 4 6例复杂视网膜脱离手术 ,2 7例在气态下行眼内半导体激光光凝 ,其中 5例在气体联合重水下完成光凝 ;1 9例在气态下直视观察行巩膜外冷疑 ,术毕 31例玻璃体腔填充 1 6 % C3F8,1 5例填充硅油。结果 :全部病例顺利完成玻璃体切除及气态下眼内操作 ,随访 6个月至 1 5个月 (平均 1 0 .3个月 ) ,治愈率 89.1 % (4 1 / 4 6 ) ,盲目率从术前 84 .8% (39/ 4 6 )下降到术后 6月 32 .6 % (1 5 / 4 6 ) (P<0 .0 1 )。结论 :广视野镜倒像装置系统为现代玻璃体视网膜显微手术提供了一个广角视野 ,它的应用使许多高精度操作能够顺利完成 ,提高了治愈率 ,减少了盲目率 ,是手术不可缺少的重要工具     

癌症患者的特殊心理状态分析及心理医护对策

目的：研究分析癌症患者的特殊心理状态及其心理医护对策。方法：对200例癌症患者进行分型调查，从不同性别、职业、经济条件及子女态度等方面进行分析研究。结果：健康心态男性多于女性，农民多于干部和工人，经济条件好者多于经济条件差者，子女态度积极者多于子女态度消极者。病理心态则相反。结论：男性独立型性格占优势，求生欲望强烈，女性顺从型性格占优势，慈爱子女心态较重，病理心态较多，干部和工人存在一定的虚荣心理，消极心理占优势，病理心态较重，经济条件属客观因素，差者病理心态增多，子女态度消极则会加重癌症患者的病理心态。     

Influence of experimental chronic hypertension on the cerebral and renal arteries of wild cats

The influence of chronic hypertension (HT) on the cerebral and renal arteries was examined pathologically and morphometrically in wild cats without a specific genetic background. Chronic HT for 8–15 months was induced by uninephrectomy and salt‐loading, and the blood pressure was monitored for a maximum of 5 months. The grade of systolic blood pressure elevation in each cat during the monitoring period was 21–51 mmHg. Histologically, the cerebral arachnoid and medullary arteries of all hypertensive cats showed a well‐preserved medial layer, and neither loss of medial smooth muscle cells, adventitial fibrosis or fibrinoid exudation was detected. This experimental model of chronic HT in wild cats for 8–15 months induced segmental intimal elastofibrosis of the arachnoid and renal arteries, but spared the cerebral medullary artery. The parenchymal changes in the brain were negligible. Morphometrically, the arachnoid artery in control cats had a significantly thinner media than the renal artery, and the medial hypertrophy of the arachnoid artery resulting from HT occurred significantly less frequently than that of the renal artery. These findings suggest that the arachnoid and medullary arteries are relatively well protected from HT, and that this may be characteristic of cerebral arteries in general and ascribed to autoregulation.     

Non-Complement- and Complement-Activating Antibodies Synergize to Cause Rejection of Cardiac Allografts

Alloantibodies (AlloAbs) are a clinically significant component of the immune response to organ transplants. In our experimental model, B10.A (H-2a) cardiac transplants survived significantly longer in C57BL/6 (H-2b) immunoglobulin knock-out (IgKO) recipients than in their wild-type (WT) counterparts. Passive transfer of a single 50-200-microg dose of complement-activating IgG2b AlloAbs to IgKO recipients reconstituted acute rejection of cardiac allografts. Although passive transfer of a subthreshold dose of 25 microg of IgG2b or a single 100-200-microg dose of non-complement-activating IgG1 AlloAbs did not restore acute rejection to IgKO recipients, a combination of these AlloAbs did cause acute graft rejection. Histologically, rejection was accompanied by augmented release of von Willebrand factor from endothelial cells. IgG1 AlloAbs did not activate complement on their own and did not augment complement activation by IgG2b AlloAbs. However, IgG1 AlloAbs stimulated cultured mouse endothelial cells to produce monocyte chemotactic protein 1 (MCP-1) and neutrophil chemoattractant growth-related oncogene alpha (KC). TNF-alpha augmented IgG1 induced secretion of MCP-1 and KC. These findings indicate that non-complement-activating AlloAbs can augment injury to allografts by complement-activating AlloAbs. Non-complement-activating AlloAbs stimulate endothelial cells to produce chemokines and this effect is augmented in the milieu of proinflammatory cytokines.     

亨廷顿病发病新说

野生型亨廷丁减少，可能是亨廷顿病发病原因之一。     

含野生型p53cDNA重组逆转录病毒的制备

目的：制备野生型p53cDNA重组体的复制缺陷型逆转录病毒。方法：设计引物PCR扩增野生型p53cDNA，用T－A克隆的方法克隆入质粒载体pGEM-T,转化JM109，提取pGEM-T/p53重组体，双酶切得到野生型p53cDNA,然后再克隆人逆转录病毒表达载体pLXSN,转化感受态大肠杆菌 JM109，筛选pL(p53cDNA)SN重组体，脂质体法转染包装细胞AM12，G－418（geneticin)筛选，收集病毒液。结果：通过PCR和重组质粒酶切筛选出重组阳性克隆，收集含pL(p53cDNA)SN重组体的逆转录病毒。结论：含pL(p53cDNA)SN重组体的复制缺陷逆转录病毒可用于转染真核细胞等进一步研究。     

高锌与缺锌对鼠胶质瘤细胞野生型-p53 mRNA表达的影响

野生型－p53(wt-p53)作为关卡基因在DNA受损伤时使细胞停止在G1期，使受损细胞修复或发生细胞凋亡，本研究利用RT0PCR法研究高Zn^2 ,缺Zn^2 作用24小时后对鼠胶质瘤细胞wt-p53 mRNA表达的影响，取对数生长期的鼠胶质瘤细胞，在RPMI－1640常规培养液中分别加入0．3mmol/L的Zn^2 和5μmol/L的TPEN培养24h后发现0．3mmol/L的Zn^2 作用24小时抑制鼠胶质细胞wt-p53表达，5μmol/L的TPEN作用24小时鼠胶质瘤细胞wt-p53mRNA表达水平升高，这表明高Zn^2 抑制鼠胶质瘤细胞wt-p53RNA表达，缺Zn^2 可提高 鼠胶质瘤细胞wt-p53RNA表达水平。